Biology 9514
Advanced Gene Expression: testing the hypotheses of modern gene expression theory
Monday 9:30-11:30AM NCB 340
Schedule:
Jan 12 Introduction
Jan 19 lactose operon Each person will be given a textbook section
to read and consult for the model for how the lactose operon works.
You each will, without notes and with no powerpoint, use props
of your choice to illustrate what happens in a bacterial cell
grown in glucose medium and then transferred to a medium containing
lactose as a carbon source.
Jan 26 lactose operon part 2. You will repeat your prop routine
incorporating data from three papers.
Feb 2 How does the heat shock response work? Class discussion
Feb 9 Lecture on the heat shock response. Setting the debate topic.
Feb 16 FAMILY DAY
Feb 23 topics for seminar
March 2 Debate and first Seminar
March 9 Seminars
March 16 Class Cancelled Tony away
March 23 Seminars- April 13 Seminars
The student will pick a topic on gene expression from a textbook and discuss what is correct and incorrect about the textbook representation relative to the primary literature.
Marks for the course:
| Lactose operon part 1 | 10% |
| Lactose operon part 2 | 10% |
| Debate | 20% |
| Seminar | 60% |
Web site: http://instruct.uwo.ca/biology/9514-001
Jan 19 Lactose operon Part 1.
| Name | Textbook |
| Gino Berardi | Genetics From Genes to Genomes 3rd ed. Hartwell et al. |
| Stefan Rentas | Modern Genetic Analysis 2nd ed. Griffiths et al. |
| Sylvie van Trest | Concepts of Genetics 9th ed. Klug et al. |
| Dan Bath | Molecular Biology of the gene 6th ed Watson et al. |
| Alissa Bults | Genetics From Genes to Genomes 3rd ed. Hartwell et al. |
| Naghmeh Rastegar | Modern Genetic Analysis 2nd ed. Griffiths et al. |
| Shujin Dai | Concepts of Genetics 9th ed. Klug et al. |
| Xinhua (Cindy) Wang | Molecular Biology of the gene 6th ed Watson et al. |
| Bin Luo | Genetics From Genes to Genomes 3rd ed. Hartwell et al. |
| Gang (Gary) Tian | Modern Genetic Analysis 2nd ed. Griffiths et al. |
Exercise: You are assigned a specific textbook to consult for this exercise listed above. You must base your routine on this reading. In 5 minutes using props individually selected you must describe what is occurring at the lac operon regulatory region first when a cell is grown in a medium lacking lactose as the carbon source and second when a cell is grown in a medium containing lactose as the carbon source. Ignore CAP. NO POWERPOINT.
Jan 26
Below are links to three papers which you will use to explain how the lactose repressor inhibits transcription of the lactose operon, and how the repression is released upon addition of lactose.
Paper 1
Paper 2&3
Exercise: Using props explain what you found in the papers in 5 minutes. A textbook that may help for some background information is Ben Lewin's Genes.
Feb 2
Class discussion. Initiation of transcription by RNAP II.
Feb 9
Lecture on poised RNAP II. Some of the papers to be discussed.
Setting the debate topic and debating format.
Schedule for talks
Feb 16
Slack week/Family day
Feb 23
Come in to discuss your topics for your seminar
March 2
Debate
| Team 1 | Team 2 |
| Stefan | Gino |
| Dan | Rezza |
| Alissa | Sylvie |
| Robin | Gary |
| Jane | Naghmeh |
| Cindy |
First seminar 1.) Robin Luo
March 9
2.) Jane
3.) Naghmeh Rastegar
March 16
Tony is away. No class.
March 23
4.) Dan Bath
5.) Rezza
March 30
6.) Sylvie van Trest
7.) Alissa Bults
April 6
8.) Gino Berardi
9.) Xinhua (Cindy) Wang
April 13
10.) Gang (Gary) Tian
11.) Stefan Rentas